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Frank Mannuzza Phones & Addresses

  • 3 Cambridge St, Chelmsford, MA 01824 (978) 244-2495
  • Lehigh Acres, FL
  • Naples, FL
  • Burlington, MA

Publications

Us Patents

Coated Membrane For Assessing The Invasive Capacity Of A Cell

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US Patent:
6740501, May 25, 2004
Filed:
Aug 29, 2001
Appl. No.:
09/942349
Inventors:
Frank J. Mannuzza - Chelmsford MA
Paula Flaherty - Tyngsboro MA
Stephen R. Ilsley - Boston MA
Martin L. Kramer - Needham MA
Assignee:
Becton, Dickinson and Company - Franklin Lakes NJ
International Classification:
C12Q 102
US Classification:
435 29, 435 34, 4352879
Abstract:
A porous membrane is coated with a composition which includes a reconstituted and aggregated extracellular matrix derived from the Englebreth-Holm-Swarm mouse tumor, a polyol and a pH 7. 8-8. 2 buffer. The coated membrane is dried, affixed to an insert portion of an assembly and received in a well of a multiwell tissue culture plate. The invention includes a method to make the coated membrane.

Method Of Providing A Substrate With A Ready-To-Use, Uniformly Distributed Extracellular Matrix

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US Patent:
7541187, Jun 2, 2009
Filed:
Jun 6, 2002
Appl. No.:
10/164486
Inventors:
Arthur Myles - Hingham MA,
Stephen R. Ilsley - Boston MA,
Frank J. Mannuzza - Chelmsford MA,
Assignee:
Becton, Dickinson and Company - Franklin Lakes NJ
International Classification:
C12N 5/00
C12N 5/02
A01N 63/00
US Classification:
435395, 4353201, 435382, 435397
Abstract:
Method of providing a substrate with a ready-to-use uniformly distributed extracellular matrix is disclosed. This method includes applying extracellular matrix components to a substrate area; incubating the extracellular matrix components to allow polymerization thereof; freezing the polymerized extracellular matrix on the substrate area; lyophilizing the frozen extracellular matrix on the substrate area; and allowing the lyophilized extracellular matrix to warm to room temperature. Also contemplated is a cell culture apparatus having a dried uniformly distributed extracellular matrix formed by the above-mentioned method.

Modified Reconstituted Basement Membrane Composition For Assay System

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US Patent:
7608455, Oct 27, 2009
Filed:
Dec 13, 2004
Appl. No.:
11/010679
Inventors:
Min Wu - Carlisle MA,
Frank J. Mannuzza - Chelmsford MA,
Assignee:
Becton, Dickinson and Company - Franklin Lakes NJ
International Classification:
C12N 5/00
US Classification:
435402, 435395, 435397
Abstract:
A modified reconstituted extracellular matrix composition is provided herein. The composition includes an extracellular matrix and at least one exogenous component selected from heparin, fibronectin and laminin. The composition may have a basic pH. Additionally, provided herein is a cell culturing system including a substrate and a coating thereon of the composition to assess potential stimulators and/or inhibitors for their effects on various cell cultures while increasing the signal dynamic range.

Modified Reconstituted Basement Membrane Composition For Assay System

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US Patent:
8541235, Sep 24, 2013
Filed:
Sep 28, 2009
Appl. No.:
12/567996
Inventors:
Min Wu - Carlisle MA,
Frank J. Mannuzza - Chelmsford MA,
Assignee:
Corning Incorporated - Corning NY
International Classification:
C12N 5/00
US Classification:
435402, 435397, 435395
Abstract:
A modified reconstituted extracellular matrix composition is provided herein. The composition includes an extracellular matrix and at least one exogenous component selected from heparin, fibronectin and laminin. The composition may have a basic pH. Additionally, provided herein is a cell culturing system including a substrate and a coating thereon of the composition to assess potential stimulators and/or inhibitors for their effects on various cell cultures while increasing the signal dynamic range.

Modified Reconstituted Basement Membrane Composition For Assay System

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US Patent:
20050079611, Apr 14, 2005
Filed:
Aug 2, 2004
Appl. No.:
10/909636
Inventors:
Min Wu - Carlisle MA,
Frank Mannuzza - Chelmsford MA,
International Classification:
C12N005/02
US Classification:
435404000, 424426000
Abstract:
A modified reconstituted extracellular matrix composition is provided herein. The composition includes an extracellular matrix and at least one exogenous component selected from heparin, fibronectin and laminin. The composition may have a basic pH. Additionally, provided herein is a cell culturing system including a substrate and a coating thereon of the composition to assess potential stimulators and/or inhibitors for their effects on various cell cultures while increasing the signal dynamic range.

Preparation Of A Cell Culture Substrate Coated With Poly-D-Lysine

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US Patent:
59324732, Aug 3, 1999
Filed:
Sep 30, 1997
Appl. No.:
8/941473
Inventors:
Mark S. Swiderek - Gloucester MA
Frank J. Mannuzza - Burlington MA
Stephen R. Ilsley - Boston MA
Arthur Myles - Acton MA
Assignee:
Becton Dickinson and Company - Franklin Lakes NJ
International Classification:
C12M 300
C12N 1100
C12N 1108
C12N 500
US Classification:
4352891
Abstract:
A cell culture substrate is coated with a composition containing a cell adhesion promoter in a salt solution. A substrate such as plastic, glass or microporous fibers is coated with a composition containing about 5. mu. g/ml to about 1000. mu. g/ml of poly-D-lysine in an 0. 005 M to about 0. 5 M citrate or sulfate salt solution to provide about 50. mu. l to about 500. mu. l of the composition per cm. sup. 2 of substrate, and the coated substrate is rinsed to remove extraneous materials and dried to obtain a coated substrate having increased shelf-life and/or stability. The coated substrate may be sterilized by rinsing with a sterilizing medium such as ethanol.

Cell Culture Substrates And Methods Of Use

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US Patent:
57417016, Apr 21, 1998
Filed:
Jan 25, 1995
Appl. No.:
8/377887
Inventors:
Mark S. Swiderek - Gloucester MA
Frank J. Mannuzza - Burlington MA
Assignee:
Becton, Dickinson and Company - Franklin Lakes NJ
International Classification:
C12M 306
US Classification:
4352971
Abstract:
Cell culture substrates comprising dried films of native fibrillar collagen produced by a method in which collagen fibers are hydrolyzed in acid, solubilized, and reformed as gels on porous surfaces under non-physiologic salt conditions to produce large fibers with the striations characteristic of collagen fibers found in vivo. The gels are collapsed onto the porous surfaces by drawing the interfibril fluid out of the gel through the underside of the porous surface and then dried to form films. Dried collagen films made in this manner retain native fibrillar collagen structure and excellent diffusion characteristics. Native fibrillar collagen films produced according to the methods of the invention are useful as cell culture substrates. They have particularly advantageous properties for growth and differentiation of epithelial cells. This effect is synergistically enhanced by addition of butyric acid as a differentiation inducing agent.

Cholesterol-Rich Fraction Useful In Culture Media And Process Of Producing Same

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US Patent:
47627921, Aug 9, 1988
Filed:
Oct 28, 1986
Appl. No.:
6/923850
Inventors:
Makram M. Girgis - Bradley IL
David E. Jackson - Bourbonnais IL
Gerald L. Kruse - Herscher IL
Frank J. Mannuzza - Burlington MA
Joseph G. Montalto - Bradley IL
Assignee:
Miles Inc. - Elkhart IN
International Classification:
C12N 138
US Classification:
435244
Abstract:
A process is provided for isolating and purifying a cholesterol-rich fraction from mammalian blood plasma or serum. The resulting cholesterol-rich fraction is useful as a growth medium ingredient.
Frank J Mannuzza from Chelmsford, MA, age ~80 Get Report