20030021796, Jan 30, 2003
Leszek Ignatowicz - Evans GA, US
Piotr Kraj - Augusta GA, US
424/185100, 424/186100, 424/190100, 424/191100
Disclosed is an in vivo system for the development of CD4 T cells bearing class II MHC restricted TCR. The cells are induced by the administration of a positively selecting, soluble peptide. Following peptide delivery, double-positive CD4CD8 cells expressing this TCR differentiate into CD4 cells in vivo, or in vitro in thymic organ cultures. This system facilitates the development of antigen-specific functional CD4 T cells in a controlled manner, after administration of the peptide. The positively selected CD4 T cells remain in the periphery for a prolonged time and respond to the appropriate antigenic challenge.